Abstract

Lipases are a class of enzymes that catalyze the hydrolysis of long chain triglycerides in the lipids-water interface. Microbial lipases are currently receiving great attention to the rapid development of enzyme technology. Lipases have great potential in various industrial applications, chemical, pharmaceutical, medical, cosmetic, leather industry, paper manufacture, synthesis, biosurfactant, and agrochemicals. In this study, several bacteria have been tested and chosen for their ability to synthesize lipases. The aim of this research was to perform a screening of lipolytic strains as well as the dosage of their lipase activities. Gene which encodes the 16S ribosomal RNA for bacterial species was amplified via PCR and sequenced, the activity of lipase was examined within the pH range of 6.0-10.0 and the effect of pH on lipase stability was determined by incubating the lipase fraction in various buffer solutions ranging from 3.0 to 10.0 for 24 h at room temperature. The extracellular lipases from B. subtilis have a maximum production obtained after 72 hours at 50 °C, pH 8, and 40 mM of CaCl2. The results showed that the use of olive oil strongly induces the lipase production from Bacillus subtilis.